Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Outer wells are aligned for proper viewing with FLIPR. The Gemini EM Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Gemini EM and SpectraMax M2 Microplate Readers By Yan Zhang, Molecular Devices Corporation, 1311 Orleans Dr. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. With theThe StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. Detection by Low noise PMT with simultaneous digital and analog modes. This can increase throughput and efficiency, thus saving time and space in the lab. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. ) 20,000 10,000 30,000 40,000 50,000 60,000 70,000 80,000 0. the appropriate well of the microplate. Six wells of PBS were used as blanks for calculating the lower limit of detection (LLD). 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. 1 sec. Note: SoftMax Pro Software must be installed on a computer connected to the instrument. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deepEight filters enable visible light absorption measurement applications such as protein quantification, cell viability and ELISA. Fluorescent DNA quantitation. The Perkin Elmer Wallac 1420. 40266. Molecular Devices VersaMax Microplate Reader Specifications. Published Dec 16, 2020 | Updated Dec 06, 2021. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Brand: MOLECULAR DEVICES. 96-well: 0. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Infinite 200 Pro Microplate Reader. Injectors Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. Pick the best speed, sensitivity, and price for your needs. Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. Important things to consider when purchasing a multimode microplate reader. Four wash/rinse bottles are included and configurable for both 96- and 384. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. You can mix the contents of the wells in a microplate automatically by shaking before each read cycle. While they play an important role in generating data from a wide range of. 03 pM. Jon Cuccui, Brendan W. OEM Molecular Devices All Microplate Reader, Top r. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. softmaxt pro microplate data analysis, molecular devices’ stakmax microplate stacker, monochromator-based fluorimeter, wavelength optimization, fda 21 cfr part 11. Paradigm: Delta mP. The microplate reader will read the plate and the relative fluorescence units will be displayed in the Plate section. The Molecular Devices Versamax Microplate Reader INCLUDED:. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). robotics-automation-compatible. The FilterMax F5 can read 6, 12, 24, 48, 96, 384, 1536-well. Since then, the microplate measurements of DNA, RNA, and proteins that this enabled have become very popular. microplate . 76 L x 85. Please refer to the Obsolete Microplate Reader Models for more information. Molecular. The CellTiter-Glo assay from Promega uses luciferase enzyme. 采用ELISA 方法，对比Molecular Devices 与某进口品牌中等吸附和高等吸附96 孔全透明微孔板，结果表明两种品牌高等吸附板都能结合更多的Protein A，同时Molecular Devices 全透明微孔板有更优的复孔重复性。 The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. SpectraMax® 340PC384 Microplate Reader for. #ABS PLUS) Methods. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Three kits covering a wide range of dsDNA concentrations. 2. For more sample throughput, both readers can be easily integrated into full roboticPrimary considerations when choosing a microplate reader are throughput and flexibility. Flexible for 6-well to 384-well microplates and cuvettes. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity assays. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate:The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. 8. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. 00. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. ZERO BIAS - scores, article reviews, protocol conditions and moreThe reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. • 0. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. The OptiMax by Molecular Devices is a tunable microplate reader. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. 500 USD. If read values are low, try setting PMT sensitivity to High in the Settings menu of the Plate section. When the user applies a set temperature of 37°C, the top is heated to 37. In . 6:30 . Microscopy. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C overnight. Other Molecular Devices readers with absorbance detection mode, e. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate. Vrla used the device to quantify the concentration of a small molecule produced by. 96-well plates: V-bottom: 20-30 µl U-bottom: 30-40 µl flat-bottom: 50-60 µl 384-well plates: approximately 20 µl Recommended ArticlesA method is described for the routine determination of the rate of colorimetric enzyme reactions using a 96-well microtiter plate reader commonly used in. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. This is equivalent to having 511 built-in filters, Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. Monochromatic filter is equipped. personalized as your research. Both readers were heated to 37ºC prior to setting up the assay. Elisa Plate Readers and Microplate Readers at Biocompare. Materials. Plate Reader with Microplate Adapter, Molecular Devices SpectraMax M series Multi-mode 6. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn moreThe Molecular Devices microplate reader portfolio comprises various instruments, which differ regarding the design of the heating elements, as well as temperature control, and are therefore divided into three categories as shown in Table 1. Taking into account the price differences between each seller and each country. i3x. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. • 0. Workflows of the MTT Cell Proliferation and Cell Cytotoxicity Assay. Endpoint, kinetic, spectrum and area-well scanning Click here. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. 384-well: 0. 0. StakMax. 1/17 1907B Printed in USA . System setup and. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. Vmax Kinetic Microplate Reader with SOFTmax® Pro from Molecular Devices, now part of MDS Analytical Technologies. The absorbance of the tested solutions was measured at 405 nm using a SpectraMax® ABS PLUS Absorbance Microplate Reader (Molecular Devices, LLC, Sunnyvale, CA, USA). Cells were loaded with 2 µM Fluo-4 for one hour. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aSpectraMax Microplate Readers. Transfer of an assay to 96-well microplate technology increases throughput significantly. for ValitaTiter Plus standards read on Molecular Devices readers. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. “The ABS Plus reader comes standard with our patented PathCheck® Technology which enables consistent results across a 96- or 384-well plate. 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. We would like to show you a description here but the site won’t allow us. This instrument is ideal for measuring dual luciferase assays in both 96- and 384-well plates, and SoftMax Pro® software simplifies data collection and analysis with preconfigured protocols. Read time* 96 wells < 15 sec. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. A FlexStation 3 Microplate reader ( Molecular Devices; San Jose, USA) was used to take fluorescence readings every 2 s (excitation 485. If the best wavelengths are unknown, the Gemini EM reader will find. , 384 wells < 45 sec. Related Products: Low Binding Plates. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. Step 2: Launch SoftMax Pro Software. The system provides automation for up to 50 microplates for easy walkaway automation. Simple, economical . 03 pM. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. 14: Average %CV. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. Triplicates of each BSA concentration were made for this assay plate. Compare this item. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. 1-color 96-well . Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads the VMax Microplate Reader has both speed and performance. #SpectraMax L Config) Methods. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs) Specifications/Features: Shaking, Temperature control, Injector. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs). Molecular Devices i3x. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. and reliable microplate stacking solution for Molecular Devices microplate readers and washers. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. FlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Click “Next”, and the reader will start scanning the four corner wells; a progress indicator will appear: fter the reader finishes scanning the four corner wells, you will see a screen with an image of the upper left corner A well and a pink crosshair. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. +49-89/96-05-88-0 Fax +49-89/9-62-02-34-5 Japan. Model:. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. fluorescence microplate reader from Molecular Devices. 7°C (gradient heating design). #N207) LAL Reagent Water (Lonza cat. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. #VAL003 or VAL013) or ValitaTiter Plus (Molecular Devices cat. # 0310-5217T) 384-well cell wash head (Molecular Devices Cat. SpectraMax ABS Plus reader utilizes Molecular Devices PathCheck Sensor- the only patented. Protein quantitation Cross-talk < 0. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. This reader's dual monochromators for. It includes both shaking and incubation functions, and is compatible with 96- well microplates. Seller assumes all responsibility for this listing. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. Working in microplates allows for smaller assays that use less sample and reagent and increases throughput. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). #T-0254) Lysozyme (Sigma cat. the SpectraMax i3x Multi-Mode Microplate Reader, may also be used for this application. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices cat. 5°C at 37°C. The plate reader optics are tunable for. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. Other technologies such as imaging. The FilterMax F3 can read 96-well and 384-well plates. The iMark. For more sample throughput, both readers can be easily integrated into full robotic Microplate Readers. Step 1: Turn on your microplate reader. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). *Simple analysis and optimization. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. However, an upgradeable multi-mode reader which includes absorbance, fluorescence and luminescence can address all your future lab needs. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. #I2511) • Assay diluent – may be cell culture medium. The IC design and microplate construct represents a major advancement to state-of-the-art CMOS devices for biological measurement, where the large majority are. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. both cuvette-based and microplate reader applications SpectraMax Mini Multi-Mode Microplate Reader. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. Can be integrated with Molecular Devices' StakMax® Microplate Stacker. Molecular Devices’ SpectraMax® Plus 384 Microplate Reader provides fast and reliable full spectral range detection for cuvettes, 96-well and 384-well microplates. Read one sample or up to 384 in a. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. Cross-talk < 0. F3/F5 Multi-Mode Microplate ReadersBulk Kit (10-plate size, Molecular Devices P/N R8347) HeLa cells (ATCC P/N CCL-2) Staurosporine (Sigma P/N S5921) 96-well black, clear-bottom microplates (Corning P/N 3904) SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn more Plate chamber design of the SpectraMax category 1 microplate readers. Key applications: ELISA, nucleic acid, endotoxin testing. 384 well polystyrene microplates have plate dimensions of 127. It features a modular design that allows users to add or change detection cartridges according to their needs. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. Microplate Spectrophotometers. Our SpectraTest® ABS2, FL1, LM1,The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 µL per well. No additional manuals are available. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. Published Dec 16, 2020 | Updated Dec 16, 2020. Molecular Devices SpectraMax 384 Plus Microplate Reader. #iD5-STD) Methods ATP standard curve. Or scan up to 384 wells at any time to determine the best assay conditions. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. SpectraMax Mini Multi-Mode Microplate Reader Afordable multi-mode microplate reader with best-in-class data analysis software Perform your favorite applications including. Plate type 96-well standard clear bottom: Timing Total run time: 01:40:00: Interval: 00:01:00 More settings : Carriage speed: Slow:The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SpectraMax Plus 384 and 340PC 384 readers can accommodate standard 96-well, 384-well, and strip-well microplates. is required for optimum performance with standard 96-well and 384-well format microplates for all top-read applications. The contents of the SpectraDrop Microplate kit depend on kit type. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. absorbance, fluorescence, and luminescence with the added functionality of. (FP) configured microplate readers. Determine DNA concentrations precisely and . Filters: Excitation and emission slides for 4 filters each. All stand-alone instrument functions can be controlled using the software. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. #M5e) Methods Microplate reader compatibility test. No additional manuals are available. EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Learn how Microplate Washer configured for both 96- and 384-well plates that help scientists by providing transformational workflow into practice, to automate a tedious process, to do more with less. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:Both the MTT and Cell Cytotoxicity assays were read on the SpectraMax® ABS Plus Microplate Reader, which can read a 96-well microplate in less than 10 seconds. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of Multi-mode microplate readers with fluorescence detection. The official tips for the FlexStation® 3 Series Multi-Mode Microplate Readers system. Please refer to the Obsolete Microplate Reader Models for more information. For more sample throughput, both readers can be easily integrated into full roboticVisionMate 96 (Vial Rack Bottom Label 2D Reader) Ziath: Datapaq Express (Vial Rack Bottom Label 2D Reader). In addition, SoftMax Pro Software Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. The EMax Plus reader measures flat and round 96-well microplates. Microplate luminescent reader assay for ERK activity. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Total volume (except deep. Growth medium was then replaced with fatty acid uptake solution. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer Service or your authorized service representative. With various on-board features and capabilities,. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. The StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. Despite its form, the Absorbance 96 delivers precise and accurate results at an affordable price. 76 L x 85. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Compact design Minimum volume and. Omnia® Compatible Microplate Reader Documentation Version No. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . < 0. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. Once the assay runs its course, the final absorbance readout will take place on a SpectraMax® iD5 Multi-Mode Microplate Reader. 3. The VersaMax reads 96-well microplates. I have used Molecular Devices’ Vmax 96-well microplate readers since about the time of their inception in the mid to late-1980s. Tecan Infinite 200 Pro M Plex Microplate Reader Unit2 - AV. This reader provides visible. #SpectraMax L Config) Methods. 1 OD at 230 nm. g. #G7294). Molecular Devices has provided scientists with . The system provides options for 20-, 40-,. Look for a washer which can handle a variety of plate types, including deep well plates and 96-, 384- and 1536-well plates. Plate was read with SpectraMax 190 absorbance microplate reader (Molecular Devices, San Jose, CA, USA) using the. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. 0 GB Brand Hewlett-Packard Hard driveThe microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. Making it possible to perform kinetic analysis of solid. reagent basin for ease of pipetting into. LUMINESCENCE MICROPLATE READING: Molecular Devices: SpectraMax L: Thermo Fisher Scientific: Luminoskan Ascent:. The EMax Plus Microplate Reader measures 96-well plates and comes with eight standard filter modes to cover the entire visible range, for applications such as protein quantification, cell viability, cell proliferation and ELISA. for ValitaTiter Assays. The concentration of total free thiols in the protein. i3x. The microplate washer automatically washes all wells of the assay plate, using the desired wash program to completely remove unbound reagents and eliminate background noise. The reader is ideal for measuring flash and glow assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer (BRET), and acridinium ester flash assays, in both 96- and 384-well plates. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. 7°C and the bottom to 36. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. • SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. (FP) configured microplate readers. Up to 70% savings in overall assay time can be gained when using the Cell Cytotoxicity assay (Figure 1). , Sigma cat. changes in OD 600 were measured by periodically transferring 100-µL aliquots of each bacterial culture to a single well of a 96-well microplate and measuring absorbance at 600 nm using the SpectraMax iD3 reader and SoftMax Pro Software. Flexibility to read a variety of plate formats. 1 OD at 230 nm. Microplate Readers. Measure assay absorbance values quickly with minimal background noise on the SpectraMax iD5 reader. You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. for ValitaTiter Plus standards read on Molecular Devices readers. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. 100 fmol 1536-well (8 µL) 0. Instrument settings are listed in Table 1. 96-well microplate; Immunoassay analyzer;VersaMax price. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Figure 2. 300 µL of Detergent Compatible Bradford reagent was added to each well. in space. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro. Endpoint, kinetic, spectrum. Learn more 0200-7008 6- to 1536-well plates. Photometric and kinetic analysis mode. Temperature Control: Ambient +5C up to 42C. Molecular Devices' microplate readers. 100 fmol 384-well (75 µL) 0. i3x Multi-Mode Microplate Reader. Quantitate proteins rapidly with a 5-minute, room-temperature incubation. High. Setup Guide. SpectraMax M5eMulti-Mode Microplate Reader (Molecular Devices cat. 96-well microplate at 200 µL/well. HPV Serology Laboratory Standard Operating Procedure . Streamline BCA-based protein quantitation on the SpectraMax iD5 reader. spectramax i3x injector cartridge, smartinject technology, atp a, spectramax i3x multi-mode microplate reader, luminescent cell vi, spectramax i3x multi mode microplate reader. SpectraMax® Microplate Reader Suite of Products Knowledge Base. Retrieving. Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. 46Click the Read button in the SoftMax Proplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Microplate Reader, Molecular Devices Optimax, Refurbished. EarlyTox Live/Dead Assay Kit (Molecular Devices cat. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and UV-visible absorbance. 96-well microplate at 200 µL/well. These robust readers have been placed in labs from Antarctica to. SpectraMax 190 and VersaMax readers can accommodate standard 96-well and strip-well microplates. Both the MTT and Cell Cytotoxicity assays were read on the SpectraMax® ABS Plus Microplate Reader, which can read a 96-well microplate in less than 10 seconds. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. Limit of Detection: < 20 attomol ATP per well Dynamic range > 9 orders of magnitude. (a) HCT116 cells expressing hyBRET-ERK were plated in 96-well plates and treated with serially diluted. Supplier: Molecular Devices. This reader also makes use of a unique temperature-independent methodology for path length correction. Other features and benefits include: Eliminate changing out filters. HeLa cells were plated at 15,000 cells per well in 100 µL of medium in a black, clearbottom microplate. the appropriate well of the microplate. 92: 88. Supplementary Information. 5 mm. In addition, the iD5 reader measures TRF and FP and. Microplate Readers. The direct transfer capability reduces reagent consumption and allows for more assay conditions to be explored in a single microplate, making the system more amenable to agonist and antagonist assay formats. 3 OD at 200 nm. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular.